Figure 5. hTERT promotes EMT through the ZEB1 pathway.
A. ZEB1 knocks down in HCT116 cells. B. Western blot of E-cadherin, N-cadherin and vimentin after hTERT overexpression and ZEB1 knock down in HCT116 cells. C. The relative luciferase activity of the E-cadherin promoter in NC-HCT116, hT-HCT116 and shZ-hT-HCT116 cells. D. PCR quantification of the E-cadherin promoter at −1959and −1954 immunoprecipitated in ChIP assays from HCT116, hT-HCT116 and shZ-hT-HCT116 cells with Abs against hTERT andthe respective control IgG. Values represent relative binding to input.