Figure 1. Everolimus leads to inhibition of clonogenicity and proliferation, whereas acute apoptosis is not induced.
A. Upper images show representative wells of 6-well plates, lower bars demonstrate the decrease of colonies after 7 days (ACHN, A704: 14 days) of treatment with 1 μM Everolimus (mean ±SD; n = 3, *p < 0.05, ***p < 0.01; t-test). B. RCC cells were treated with Everolimus (1μM) for 48h and subjected to BrdU assays (mean ±SD; n = 3, *p < 0.05, **p < 0.01; t-test). C. FACS analysis shows no significant difference in cell viability after 48 h of treatment with 1μM Everolimus (mean ±SD; n = 3).