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. 2015 Nov 16;7(1):473–489. doi: 10.18632/oncotarget.6337

Figure 7. NF-κB directly binds to and up-regulates ErbB3 promoter.

Figure 7

A–E. HEK293T cells were transfected with, control or ErbB3 promoter-luciferase with or without mutations at putative κB binding sites 1, 2, and 3 (A) HEK293T cells were transfected with control or ErbB3 promoter-luciferase and indicated doses of myc-p65 or Sp-1 (B) Hep3Bx cells were transduced with p65 shRNA (C) or were treated with PDTC (D) or BAY-11–7802 (E) for 48 hr after transfection with ErbB3 promoter-luciferase. The culture medium was prepared for detecting promoter activity and the total lysates were harvested for renilla activity. F. Total lysate of Hep3B and Hep3Bx were harvested for chromatin IP analysis with anti-p65 antibody, and the binding activity of p65 on ErBb3 promoter was determined by qPCR analysis. Statistical analysis was performed by Student's t test. *, p < 0.05; **, p < 0.01 as compared to control group. G. Diagrammatic illustration of the hypothetical model derived from this study.