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. 2015 Nov 13;7(1):509–523. doi: 10.18632/oncotarget.6312

Figure 1. 4.1N expression levels in NSCLC cell lines and primary tumors.

Figure 1

(A) Protein expression level of 4.1N was analyzed in four NSCLC cell lines with different metastatic abilities. 4.1N expression was decreased in metastatic tumor cell lines (lane 1–3) compared with low metastatic 95C cells (lane 4). (B) Representative images of immunohistochemical staining for 4.1N expression. NSCLC specimens and normal lung tissues were stained for 4.1N by an anti-4.1N HP antibody. The brown color represents positive staining for 4.1N expression. Cell nuclei were counterstained with hematoxylin (blue). The normal tissue showed an intense immunoreactivity for 4.1N. For tumor tissues, more intense staining for 4.1N was observed in well differentiated tumors compared with moderately and poorly differentiated tumors. Original magnification: × 40 and × 40.