Skip to main content
. 2015 Nov 13;7(1):509–523. doi: 10.18632/oncotarget.6312

Figure 5. Effects of 4.1N on the expression of JNK-c-Jun signaling in NSCLC cell lines.

Figure 5

(A) Upon the change in 4.1N expression, expression analysis was performed of correlative JNK-c-Jun signaling molecules in H1299 and 95C cells by Western blotting. GAPDH served as the loading control. Moreover, Western blot analysis shows that β1 integrin expression was increased in 95C cells transfected with human-4.1N shRNA and was decreased in H1299 transfected with pEGFP-4.1N, compared with their counterparts. (B) PP1 inhibitor, calyculin A, abrogates the function of 4.1N in the negative regulation of the activity of JNK-c-Jun signaling. 48 h after transfection, H1299 and 95C cells were untreated (control) or pretreated with 2.5 nM of calyculin A for 1 h before harvesting the cell lysates; the expression of PP1 and correlative JNK-c-Jun signaling molecules were then detected by Western blotting. As H1299 is a p53-null cell line, we did not detect p53 expression in this cell line. (C and D) 48 h after transfection, H1299 cells were untreated (control) or pretreated with 2.5 nM of calyculin A for 1 h. Cells were then subjected to cell proliferation assays (C) and transwell migration assays (D) as described in the ‘Materials and methods’ section.