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. 2015 Nov 13;7(1):509–523. doi: 10.18632/oncotarget.6312

Figure 6. Effects of 4.1N on the expression and activity of PP1 in NSCLC cell lines.

Figure 6

(A) Western blotting analysis of PP1 expression in 95C cells transfected with human-4.1N shRNA or mouse-4.1N shRNA and in H1299 transfected with pEGFP-4.1N or pEGFP-C3. (B) The over-expression of 2 μg or 4 μg of pEGFP-4.1N in H1299 cells significantly increased (∼13% and ∼22%, respectively) the activity of PP1 activity. The data are shown as the mean ± SD from three independent experiments. (C) More p-JNK (normalized p-JNK/PP1 intensity was 1.9 ± 0.2) was detected in PP1 immunoprecipitates from H1299 cells that expressed pEGFP-4.1N as compared with the control (normalized p-JNK/PP1 intensity was 1). *p < 0.05.