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. 2015 Nov 22;7(1):524–537. doi: 10.18632/oncotarget.6358

Figure 3. PBLD inhibited proliferation, migration and invasion in vitro.

Figure 3

A. Expression of PBLD was detected by western blotting in the human liver cell-line HL-7702 and HCC cell-lines BEL-7402, HepG2, SK-HEP-1, Huh-7 and HCC-LM3 (top). Representative fluorescence images of Huh7 and HepG2 cell-lines stably transfected with PBLD_pEGFP-N1 or empty vector (pEGFP-N1) (middle). PBLD protein expressions of Huh7 and HepG2 were efficiently upregulated after transfection as detected by western blotting (bottom). (B., C.) PBLD overexpression inhibited cell proliferation in vitro as analyzed by the cck-8 assay and colony formation assay, respectively. D. Upregulation of PBLD in Huh7 and HepG2 resulted in inhibition of cell cycle progression as detected by flow cytometry. E. PBLD also inhibited HCC cell migration (left) and invasion (right) in vitro. (*P < 0.05, **P < 0.001).