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. 2015 Nov 13;7(1):610–621. doi: 10.18632/oncotarget.6311

Figure 3. Combination of compound 968 and erlotinib has synergized inhibitory effects on the growth of HCC827ER cells.

Figure 3

(A) Saturation density assay. Cells were treated with or without increasing concentrations of compound 968 for 6 days, trypsinized and counted. The data represent the average of three independent experiments (mean ± SD). *P < 0.05, **P < 0.01. (B) HCC827 and HCC827ER cells were cultured in RPMI 1640 supplemented with 10% FBS, and were treated with compound 968 (10 μM), or erlotinib (1 μM), or compound 968 (10 μM) combined with erlotinib (1 μM) for 48 hours or untreated. Cell growth was determined by crystal violet staining. Data represent the average of three independent experiments (mean ± SD). ***P < 0.001. (C) HCC827ER cells were cultured in RPMI 1640 supplemented with 10% FBS, and were treated with erlotinib (1 μM) combined with increasing concentrations of compound 968 (5, 10, 20, and 40 μM), or treated with compound 968 (10 μM) combined with increasing concentrations of erlotinib (0.5, 1, 2, and 4 μM) for 48 hours or untreated. Cell growth was determined by crystal violet staining. Data represent the average of three independent experiments (mean ± SD). *P < 0.05, **P < 0.01. (D) Time course experiment. HCC827ER cells were cultured in RPMI 1640 supplemented with 10% FBS, and were treated with compound 968 (10 μM), or erlotinib (1 μM), or compound 968 (10 μM) combined with erlotinib (1 μM) for different time or untreated. Cell growth was determined by crystal violet staining. Data represent the average of three independent experiments (mean ± SD). *P < 0.05, ***P < 0.001.