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. 2015 Oct 19;7(1):622–637. doi: 10.18632/oncotarget.5807

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Copyright: © 2016 Thorenoor et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Hierarchical clustergram discriminating tumor and non-tumor tissue of CRC patients according to differentially expressed lncRNAs (yellow color indicates tumor samples of CRC patients, blue paired non-tumor colonic tissue, P < 0.01). B. Relative expression of ZFAS1 in CRC tissues (n = 119) compared with corresponding non-tumor tissues (n = 119). ZFAS1 expression was examined by real-time PCR. (P value < 0.001). C. The relative expression level of ZFAS1 in HCT116+/+, HT-29, DLD-1, Colo-206, CaCO-2, SW-837 and SW-620 cells was determined by real-time PCR. (C) The northern blot analysis to measure the ZFAS1 expression in CRC cells HCT116+/+, DLD-1, SW-620 and HCT116−/−. D. Based on the COADREAD, Illumina HiSeq-based TCGA dataset, expression levels of ZFAS1 are significantly higher in CRC tumor tissue in comparison to non-tumor colonic tissue (P < 10−11).

A. Hierarchical clustergram discriminating tumor and non-tumor tissue of CRC patients according to differentially expressed lncRNAs (yellow color indicates tumor samples of CRC patients, blue paired non-tumor colonic tissue, P < 0.01). B. Relative expression of ZFAS1 in CRC tissues (n = 119) compared with corresponding non-tumor tissues (n = 119). ZFAS1 expression was examined by real-time PCR. (P value < 0.001). C. The relative expression level of ZFAS1 in HCT116+/+, HT-29, DLD-1, Colo-206, CaCO-2, SW-837 and SW-620 cells was determined by real-time PCR. (C) The northern blot analysis to measure the ZFAS1 expression in CRC cells HCT116+/+, DLD-1, SW-620 and HCT116−/−. D. Based on the COADREAD, Illumina HiSeq-based TCGA dataset, expression levels of ZFAS1 are significantly higher in CRC tumor tissue in comparison to non-tumor colonic tissue (P < 10−11).