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. 2015 Nov 13;7(1):638–655. doi: 10.18632/oncotarget.6313

Figure 3. Efficacy of SET antagonist, FTY720 and EMQA, for the treatment of NSCLC.

Figure 3

(A, B) EMQA was more potent against NSCLC than FTY720. (A) The viabilities of NSCLC cells after exposure to EMQA and FTY720 at indicated doses for 72 hours were determined by MTT. Point: mean, error bar: S.D. (n = 6) (B) The percentage of apoptotic and necrotic cell death induced by EMQA and FTY720 at indicated doses for 48 hours were determined by Annexin-V/PI staining. Block bar: mean of apoptotic cell, gray bar: mean of necrosis cell. (n = 3) (C) The PP2A activities of NSCLC cells were determined after exposure to EMQA 5 μM and FTY720 10 μM for 24 hours. Bar, mean; error bars, S.D. (n = 3) (D) EMQA and FTY720 induced downregulation of p-Akt and apoptosis of NSCLC cells. Three different NSCLC cell lines exposed to EMQA or FTY720 at indicated doses were analyzed by western blot. Representative western blot images of three identical experiments are shown.