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. 2015 Nov 26;7(1):700–711. doi: 10.18632/oncotarget.6396

Figure 3. Forced BAG3 expression suppressed the PPP in HCCs.

Figure 3

(A) Empty or BAG3-transdued HCCs were cultured in medium containing [2–13C] glucose, and the PPP flux was analyzed using NMR. (B) HCCs were transduced with retrovirus containing empty or BAG3 construct for 48 h. de novo DNA synthesis was analyzed using EdU incorporation. (C–D) HCCs were transduced with retrovirus containing empty or BAG3 construct for 48 h. Cellular NADPH (C) levels and NADPH/NADP+ ratio (D) were measured and normalized by cellular proteins.