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. 2015 Nov 26;7(1):712–728. doi: 10.18632/oncotarget.6412

Figure 8. RSK1 and RSK2 silencing affects FN expression and TGF-β1 secretion by impairing YB-1 phosphorylation.

Figure 8

A. Western blot analysis of YB-1 phosphorylation in OVCAR-8 cells silenced and rescued as described in the Legend to Figure 1; RSK1/2 band intensity was quantified versus vinculin; P-YB-1 versus YB-1 B. Western blot analysis of fibronectin (FN) and SNAIL in cells where YB-1 expression was suppressed with two different shRNAs; C. measurement of TGF-β1 secretion using MSD technology in cells silenced as in the panel (A) and (B); D–E. Quantitative Real-time PCR to evaluate the expression at mRNA level of the listed genes in cells silenced as indicated. All blots were re-probed with vinculin antibody to confirm equal loading.