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. 2015 Nov 24;7(1):745–758. doi: 10.18632/oncotarget.6378

Figure 5. Effect of PTC-209 on mRNA expression of BMI1 and RING1B and on protein levels of BMI1 and H2AK119ub.

Figure 5

A. Changes of BMI1 and RING1B mRNA levels after 72 h PTC-209 treatment (1.25 μM) in GBC cells. Data were normalized to BETA-ACTIN and related to untreated controls (n = 4 for BMI1, n = 3 for RING1B). B. Quantification of changes in protein levels of BMI1 and H2AK119ub in GBC cells after treatment with 1.25 μM PTC-209 for 72 h using the ImageJ software (n = 4). C. Western blot images of GBC cells treated with 1.25 μM PTC-209 for 72 h (n = 4, cropped). Abbreviations: BMI1: BMI1 polycomb ring finger oncogene; h: hours; H2AK119ub: mono-ubiquitylation of histone 2A at lysine 119; PRC1: polycomb repressive complex 1; RING1B: ring finger protein 2.