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. 2015 Nov 9;7(1):798–813. doi: 10.18632/oncotarget.5985

Figure 6. POSTN promoted proliferation of fibroblasts via autocrine or paracrine modes of action.

Figure 6

A. the effect of CRC cell conditional media on POSTN mRNA transcription in CCD-18Co colonic fibroblasts, as measured by qRT-PCR. B. the effect of CRC cell conditional media on POSTN protein production in CCD-18Co cells by western blot analysis. C. proliferation of CCD-18Co cultured with RKO conditional medium and CCD-18Co with normal complete medium. D. proliferation of CCD-18Co-POSTN, CCD-18Co-NC, and parental CCD-18Co cells. E. migration of CCD-18Co-POSTN and CCD-18Co-NC cells in the Transwell chamber without matrigel. F. Invasion of CCD-18Co-POSTN and CCD-18Co-NC cells in the Transwell chamber with matrigel. G. anchorage independent growth of CCD-18Co-POSTN and CCD-18Co-NC cells and the effect of the signaling inhibitor LY294002 or XAV939 on POSTON-upregulated anchorage independent growth and expression levels of pAkt and β-catenin in colonic fibroblasts. Abbreviations and marks: CRC, colorectal carcinoma; pAkt, phosphorylation of Akt; qRT-PCR, real-time quantitative RT-PCR; •, P > 0.05; ***, P < 0.001.

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