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a. Knockdown of GDF15 in SW620 cells using shRNA; upper panel represents RT-PCR and lower panel represents immunoblotting. b. Migration (upper panel) and invasion (lower panel) assay for SW620 with GDF15 shRNA (quantification in right panels). c. Immunofluorescence assay for SW620 with GDF15 shRNA. d. Immunoblotting assay for expression of E-cadherin, vimentin and Twist in GDF15 knockdown SW620 cells.
