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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Prog Polym Sci. 2015 Mar 28;53:86–168. doi: 10.1016/j.progpolymsci.2015.02.004

Fig. 22.

Fig. 22

Representative scanning electron microscopy (SEM) images of cell sheet formation. Bone marrow mesenchymal stem cells were plated into 6-well plates at a density of 1 × 106 cells/well and cultured until the cells reached 100% confluence, after which they were induced to form cell sheets using cell-sheet-inducing medium (i.e., basal medium supplemented with 50 μg/mL vitamin C) for a 10-day period. (A) Three days following cell sheet induction: extracellular matrix protein production; (B) 5 days following cell sheet induction: cell–cell and cell–matrix interactions established; (C) 5 days following cell sheet induction: sheet-like structure formation; (D) 10 days following cell sheet induction: complete cell sheet formation.