Fig. 5.

NO donors increase the occurrence of spontaneous locomotor activity at pro-metamorphic stages. A: VR recording from a stage 54 tadpole both prior to, and during, NO donor [S-nitroso-N-acetyl penicillamine (SNAP), 200 μM] application and then following washout with expanded excerpts of L/R alternating VR (L/R 16) bursting during fictive swimming in each condition. Bi: pooled data showing the mean percent time spent active in each condition (n = 13). Bii: pooled data from the same preparations [as illustrated in expanded excerpts in A showing mean BD, CP, and ED (see Fig. 3Aii)] relative to control values during and after SNAP application. C: same pooled data analyses as in B, but for application of a different NO donor, DEA-NO (50–200 μM; n = 14). Values are means ± SE. *P < 0.05.