Table 1.
SNAP |
DEA-NO |
l-NAME |
PTIO |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Tissue Exposed/Parameter | Control | Drug | Wash | Control | Drug | Wash | Control | Drug | Wash | Control | Drug | Wash |
Whole preparation | ||||||||||||
Swim, % | 4.25 ± 2.03 | 14.33 ± 5.82 | 7.34 ± 3.42 | 1.06 ± 0.34 | 3.01 ± 0.72 | 1.73 ± 0.39 | 10.42 ± 1.60 | 4.33 ± 0.88 | 7.91 ± 1.52 | 4.62 ± 1.60 | 1.37 ± 0.37 | 3.52 ± 10.65 |
Burst duration, ms | 82.40 ± 7.66 | 89.39 ± 8.87 | 89.07 ± 7.93 | 128.20 ± 33.99 | 107.50 ± 21.29 | 109.81 ± 19.93 | 70.35 ± 7.69 | 79.54 ± 9.19 | 73.00 ± 5.16 | 70.90 ± 7.13 | 91.02 ± 18.85 | 82.38 ± 12.29 |
Cycle period, ms | 195.68 ± 11.18 | 223.92 ± 15.97 | 200.56 ± 13.19 | 211.78 ± 16.24 | 213.27 ± 11.02 | 217.45 ± 17.88 | 196.57 ± 7.58 | 204.31 ± 12.47 | 198.56 ± 11.66 | 197.88 ± 15.10 | 207.19 ± 17.97 | 206.01 ± 14.87 |
Episode duration, s | 4.25 ± 2.03 | 14.33 ± 5.82 | 7.34 ± 3.42 | 1.06 ± 0.34 | 3.01 ± 0.72 | 1.73 ± 0.39 | 10.42 ± 1.60 | 4.33 ± 0.88 | 7.91 ± 1.52 | 5.23 ± 1.15 | 5.03 ± 1.28 | 3.66 ± 0.61 |
SNAP and DEA-NO |
l-NAME and PTIO |
|||||
---|---|---|---|---|---|---|
Control | Drug | Wash | Control | Drug | Wash | |
Spinal cord | ||||||
Swim, % | 2.92 ± 2.02 | 2.46 ± 1.83 | 1.02 ± 0.49 | 1.15 ± 0.31 | 0.86 ± 0.14 | 0.90 ± 0.18 |
Burst duration, ms | 89.42 ± 12.09 | 96.17 ± 18.50 | 85.00 ± 7.67 | 90.64 ± 12.77 | 107.01 ± 19.95 | 90.70 ± 13.61 |
Cycle period, ms | 252.35 ± 13.67 | 268.73 ± 32.86 | 239.38 ± 18.00 | 206.64 ± 18.25 | 220.85 ± 21.37 | 186.63 ± 17.24 |
Episode duration, s | 3.46 ± 0.98 | 2.38 ± 0.44 | 2.55 ± 0.49 | 2.49 ± 0.79 | 2.56 ± 0.89 | 3.40 ± 1.10 |
Brain stem | ||||||
Swim, % | 1.04 ± 0.48 | 1.61 ± 0.44 | 1.01 ± 0.40 | 3.97 ± 3.14 | 0.44 ± 0.20 | 3.59 ± 2.86 |
Burst duration, ms | 85.00 ± 7.67 | 98.26 ± 12.68 | 81.60 ± 6.08 | 93.39 ± 11.81 | 110.98 ± 18.73 | 83.99 ± 8.68 |
Cycle period, ms | 239.38 ± 18.00 | 251.09 ± 24.12 | 220.50 ± 11.86 | 168.96 ± 11.90 | 178.18 ± 17.90 | 180.46 ± 7.87 |
Episode duration, s | 2.49 ± 0.49 | 2.22 ± 0.28 | 3.11 ± 0.85 | 2.59 ± 0.90 | 2.44 ± 1.53 | 2.57 ± 0.77 |
Values are means ± SE. SNAP, S-nitroso-N-acetyl penicillamine; DEA-NO, diethylamine NONOate; l-NAME, Nω-nitro-l-arginine methyl ester; PTIO, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide.