TABLE 1.

Mutations associated with reduced susceptibility to LZD in 32 mutants of M. tuberculosis

Straina	rrlb	rplCb	RplC amino acid change	Mutation percentage	Growth on various concentrations of LZD (μg/ml)c
					0	0.25	0.5	1	2
H37Rv	(-)	(-)			4+	-	-	-	-
L1T5-3	G2270C	(-)			4+	4+	3+	2+	-
L1T1-5	G2270T	(-)			4+	4+	4+	3+	-
L1T3-1	G2270T	(-)			4+	4+	3+	2+	-
*L1T3-4	G2270C	(-)		20	4+	4+	2+	1+	-
L1T5-4	G2270T	(-)			4+	3+	3+	2+	-
*L1T5-5	G2270T	(-)		6	4+	3+	2+	1+	-
L1T4-3	G2270T	(-)			ND	ND	ND	ND	ND
L1T4-6	G2746A	(-)			ND	ND	ND	ND	ND
L1T4-7	G2746A	(-)			ND	ND	ND	ND	ND
L1T13-2	G2746A	(-)			ND	ND	ND	ND	ND
L1T6-3	G2746A	(-)			4+	3+	3+	1+	-
L1T4-4	G2746A	(-)			4+	4+	3+	2+	-
L1T4-5	G2746A	(-)			4+	4+	3+	2+	-
L1T4-8	G2746A	(-)			4+	4+	2+	2+	-
L1T4-9	G2746A	(-)			4+	4+	2+	1+	-
L1T1-6	A2810T	(-)			4+	3+	2+	1+	-
*L1T3-6	C2848A	(-)		13	4+	2+	2+	1+	-
L1T3-2	(-)	T460C	Cys154Arg		4+	4+	4+	4+	4+
L1T4-2	(-)	T460C	Cys154Arg		4+	4+	4+	4+	3+
L1T5-2	(-)	T460C	Cys154Arg		4+	4+	4+	4+	4+
L2T14-1	(-)	T460C	Cys154Arg		4+	4+	4+	4+	4+
L2T15-1	(-)	T460C	Cys154Arg		4+	4+	4+	4+	4+
L2T15-3	(-)	T460C	Cys154Arg		4+	4+	4+	4+	3+
L2T15-4	(-)	T460C	Cys154Arg		4+	4+	4+	4+	3+
L2T15-5	(-)	T460C	Cys154Arg		4+	4+	4+	4+	3+
L2T15-6	(-)	T460C	Cys154Arg		4+	4+	4+	4+	3+
L2T22-1	(-)	T460C	Cys154Arg		3+	3+	3+	2+	2+
L2T22-2	(-)	T460C	Cys154Arg		4+	4+	4+	4+	4+
*L1T3-3	(-)	T460C	Cys154Arg	17	4+	4+	4+	4+	3+
*L1T5-1	(-)	T460C	Cys154Arg	60	4+	4+	4+	4+	4+
*L1T3-5	(-)	T460C	Cys154Arg	27	4+	4+	4+	4+	3+
*L1T3-7	(-)	T460C	Cys154Arg	17	4+	4+	4+	4+	3+

^aStrains beginning with asterisks were subjected to whole-genome sequencing.

^b(-), no mutation.

^c4+, robust growth; 3+, good growth; 2+, moderate growth; 1+, poor growth; -, no growth; ND, not determined.