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. 2016 Mar 25;60(4):2373–2382. doi: 10.1128/AAC.02840-15

TABLE 3.

MICs of antibiotics, biocides, and metals for ECL13047 (wild type), ΔacrB, and ΔacrB transcomplemented strains carrying plasmids containing the different putative RND drug transporter ORFs

Compounda MIC (μg/ml) for strainb:
ECL13047 ΔacrB ΔacrB/ECL_01233-1234 ΔacrB/ECL_00053-55 ΔacrB/ECL_01758 ΔacrB/ECL_01960-63 ΔacrB/ECL_02124-25 ΔacrB/ECL_02243-44 ΔacrB/ECL_03149-50 ΔacrB/ECL_03401-04 ΔacrB/ECL_03767 ΔacrB/ECL_04649-50 ΔacrB/ECL_04888-93
Antibiotics
    Piperacillin 4 4 4 4 4 4 4 4 4 4 4 4 4
    Cefotaxime 2 2 2 2 2 2 2 2 2 2 2 2 2
    Cefoxitin 256 256 256 256 256 256 256 256 256 256 256 256 256
    Cefepime 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032
    Imipenem 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
    Ertapenem 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032 0.032
    Gentamicin 0.125 0.25 0,125 0.25 1 (4) 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
    Tobramycin 0.5 0.25 0,125 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0,25 0.25
    Amikacin 1 1 1 0.5 4 (4) 0.5 0.5 1 1 1 1 0.5 0.5
    Norfloxacin 0.125 0.032 0.125 (4) 0.125 (4) 0.125 (4) 0.032 0.5 (16) 0.032 0.032 0.032 0.032 0.032 0.032
    Levofloxacin 0.064 0.016 0.064 (4) 0.25 (16) 0.125 (8) 0.016 0.25 (16) 0.032 0.008 0.008 0.016 0.032 0.032
    Ciprofloxacin 0.032 0.008 0.032 (4) 0.064 (8) 0.064 (8) 0.016 0.125 (16) 0.016 0.004 0.008 0.016 0.016 0.016
    Moxifloxacin 0.064 0.008 0.125 (16) 0.125 (16) 0.25 (32) 0.008 0.125 (16) 0.008 0.008 0.008 0.008 0.016 0.008
    Tetracycline 2 0.5 1 2 (4) 4 (8) 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
    Tigecycline 0.5 0.125 0.5 (4) 1 (8) 2 (16) 0.125 0.125 0.125 0.064 0.064 0.125 0.125 0.125
    SXT 2 0.5 2 (4) 0.5 0.5 0.5 32 (64) 0.5 0.5 0.5 0.5 0.5 0.5
    Chloramphenicol 8 0.5 4 (8) 8 (16) 2 (4) 1 32 (64) 1 1 1 1 1 1
    Fusidic acid 256 4 256 (64) 256 (64) 64 (16) 4 16 (4) 4 8 32 (8) 64 (16) 4 4
    Nitrofuratoin 32 32 32 32 32 32 64 32 64 32 32 64 32
    Erythromycin 256 16 256 (16) 256 (16) 256 (16) 16 16 16 16 16 32 64 (4) 16
    Colistin 256 128 128 128 128 128 128 128 128 128 128 128 128
Antiseptics
    Benzalkonium chloride 32 4 32 (8) 16 (4) 8 8 16 (4) 8 16 (4) 4 8 8 8
    CTAB 16 16 16 16 16 8 16 16 16 16 16 16 16
    Chlorhexidine 2 1 2 4 (4) 2 1 1 1 2 1 1 1 1
    Tetraphenylphosphosnium >1,024 128 >1,024 (>8) >1,024 (>8) 512 (4) 128 512 (4) 128 256 64 64 128 128
Biocides
    Acridine 512 64 512 (8) 256 (4) 64 32 128 64 32 16 32 32 32
    Acriflavine 64 8 64 (8) 64 (8) 16 8 32 (4) 8 4 4 8 8 8
    Cristal violet 32 1 16 (16) 16 (16) 1 1 4 (4) 1 1 1 1 1 1
    Ethidium bromide 1,024 64 1,024 (16) 1,024 (16) 128 64 256 (4) 32 128 256 32 32 64
    Rhodamine >1,024 128 1,024 (8) 1,024 (8) 128 128 1,024 (8) 128 128 128 128 128 128
Metals
    Copper 1,024 1,024 512 512 512 512 512 512 1,024 1,024 512 512 512
    Zinc 128 128 128 128 128 128 128 128 64 128 128 128 128
    Manganese >1,024 >1,024 >1,024 >1,024 >1,024 >1,024 >1,024 >1,024 1,024 >1,024 >1,024 >1,024 >1,024
    Silver 2 2 2 2 2 2 2 2 1 2 2 8 4
    Mercury 4 8 8 8 8 8 8 8 8 8 8 8 8
Other
    SDS 1 0.004 1 (256) 0.5 (128) 0.016 (4) 0.004 0.032 (8) 0.004 0.004 0.004 0.004 0.004 0.004
a

SXT, trimethoprim-sulfamethoxazole; CTAB, cetyltrimethylammonium bromide; SDS, sodium dodecyl sulfate.

b

Numbers in boldface are MICs that are significantly different (fold changes of ≥4 are indicated in parentheses) from the MICs of the ΔacrB mutant strain.