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Garcinol reduces levels of lysine acetylation on TgGCN5b. HATgGCN5b was immunoprecipitated from HATgGCN5b-expressing parasites after 12 h of treatment with either 5 μM garcinol (GAR) or the vehicle (DMSO). Acetylation levels of TgGCN5b were detected by using a pan-acetyl-lysine antibody. Levels of total TgGCN5b protein were determined with an anti-HA antibody. *, P < 0.05. Error bars represent standard deviations of data from three independent experiments.
