FIG 5.

Garcinol inhibits asexual replication of apicomplexan parasites. (A) A parasite counting assay was used to determine the effect of garcinol on T. gondii strain ME49 tachyzoite replication. Stacked bars represent the proportions of vacuoles containing 1, 2, 4, 8, or 16 parasites at each concentration of garcinol tested. (B) Growth inhibition curve of T. gondii RH tachyzoites treated with increasing concentrations of garcinol. The number of parasites was quantified by qPCR of the parasite-specific B1 gene. (C) Growth inhibition curves of P. falciparum strains HB3 and Dd2 treated with increasing concentrations of garcinol. Error bars represent standard deviations of data from at least three independent assays.