TABLE 1.
Parameters of the bioanalytical methods for quantification of BNZ in plasma and urine
| Parametera | Result for: |
|
|---|---|---|
| Plasma | Urine | |
| Linearity (n = 10) | ||
| Concn range, μg/ml | 0.1562–20 | 0.0390–20 |
| Correlation coefficient | r = 0.9995 | r = 0.9998 |
| Equation | y = 1.5626x − 0.1968 | y = 0.8445x − 0.0174 |
| LLOQ, μg/ml | 0.1562 | 0.0390 |
| Precision, RSD | ||
| Intra-assay | 8.18 | 10.1 |
| Interassay | 10.3 | 10.6 |
| Accuracy | ||
| Intra-assay | 93.8 | 97.3 |
| Interassay | 101.6 | 100.2 |
| Recovery, % | 65.5 | 82.0 |
| Stability, RSD/accuracy | ||
| Short term (24 h at room temp) | 3.85/94.2 | 6.5/96.5 |
| Long term (14 days at 20°C) | 5.5/107.3 | 10.0/101.3 |
| Postpreparative (24 h at 25°C) | 3.5/92.4 | 7.4/103.4 |
a
LLOQ, lower limit of quantitation; RSD, relative standard deviation.