TABLE 4.
Performance of mutation detection (gyrA codon 88 to 94 or gyrB G512R) for identification of ofloxacin and moxifloxacin resistance (low-level and high-level) in different strains of multidrug-resistant Mycobacterium tuberculosis
| Agent | Strain | No. of isolates |
Performance, % (95% confidence interval) |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Resistant |
Susceptible |
|||||||||
| Mutation positive | Mutation negative | Mutation positive | Mutation negative | Sensitivity | Specificity | LR+ | LR− | Accuracy | ||
| Ofloxacin | All | 40 | 18 | 9 | 44 | 69.0 (55.5–80.5) | 83.0 (70.2–91.9) | 4.1 (2.2–7.5) | 0.4 (0.3–0.6) | 75.7 (66.6–83.3) |
| East Asian (Beijing) | 18 | 7 | 5 | 24 | 72.0 (50.6–87.9) | 82.8 (64.2–94.2) | 4.2 (1.8–9.6) | 0.3 (0.2–0.6) | 77.8 (64.4–88.0) | |
| Euro-American | 10 | 6 | 0 | 9 | 62.5 (35.4–84.8) | 100.0 (66.4–100.0) | >10.0 | 0.4 (0.2–0.7) | 76.0 (54.9–90.6) | |
| Indo-Oceanic | 10 | 5 | 4 | 11 | 66.7 (38.4–88.2) | 73.3 (44.9–92.2) | 2.5 (1.0–6.2) | 0.5 (0.2–1.0) | 70.0 (50.6–85.3) | |
| Moxifloxacin | All | 42 | 20 | 7 | 42 | 67.7 (54.7–79.1) | 85.7 (72.8–94.1) | 4.7 (2.3–9.6) | 0.4 (0.3–0.5) | 75.7 (66.6–83.3) |
| East Asian (Beijing) | 19 | 10 | 4 | 21 | 65.5 (45.7–82.1) | 84.0 (63.9–95.5) | 4.1 (1.6–10.4) | 0.4 (0.2–0.7) | 74.1 (60.3–85.0) | |
| Euro-American | 10 | 5 | 0 | 10 | 66.7 (38.4–88.2) | 100.0 (69.2–100.0) | >10.0 | 0.3 (0.2–0.7) | 80.0 (59.3–93.2) | |
| Indo-Oceanic | 10 | 5 | 4 | 11 | 66.7 (38.4–88.2) | 73.3 (44.9–92.2) | 2.5 (1.0–6.2) | 0.5 (0.2–1.0) | 70.0 (50.6–85.3) | |