FIG 1.

Antiviral activities of different IFN types against HEV replication. The dose-dependent inhibition rates of HEV subgenomic replicon (SGR) (A, C) and HCV SGR (B, D) replication in Huh7.5 cells by representatives of all three IFN types are depicted. IFN-α2a as the type I representative, IFN-γ as the sole type II interferon, and IFN-λ3 as the IFN type III were added 4 h posttransfection. The y axes display the relative light units (RLUs) normalized to those of the untreated control, assessed 72 h posttreatment; the x axes reflect the IFN concentrations utilized. IFN-γ was used as international units per milliliter (A, B), and IFN-λ3 was implemented as nanograms per milliliter (C, D). IFN-α2a concentrations were applied based on international units per milliliter and nanograms per milliliter. Shown are the means (± standard errors of the mean [SEM]) from at least three independent experiments; asterisks represent significant reductions in RLUs tested against untreated controls (Dunnett's t test; *P < 0.05; **P < 0.01; ***P < 0.001).