FIG 3.

NO-np induces apoptosis and necrosis of C. albicans cells. (A) Yeast cells were treated with np or NO-np and compared to untreated fungal cells. Apoptotic cells were analyzed by flow cytometry after being stained with annexin V-FITC together with propidium iodide (PI). The percentages of viable (live) and apoptotic or nonapoptotic dead cells are reported. (B) Fluorescence microscopy images of untreated, np-treated, and NO-np-treated filamentous C. albicans. Representative images of fungal hyphae showed viable (no fluorescence), apoptotic (annexin V; green), and dead (PI; red) cells. The pictures were taken at a magnification of ×20. Scale bar, 20 μm. All experiments were performed twice, with similar results obtained each time.