FIG. 7.

(A) Hyperintense manganese-enhanced contrast observed in T1-weighted images arranged in a crescent shape and surrounding the lesion core on day14 from the low Mn²⁺ dose (group 2). (B) Glial fibrillary acidic protein (green) and DAPI (blue) stained glial cells and nuclei, respectively, under a 10X objective collected on a confocal microscope were taken in a portion of the lesion. (C) 20X confocal images show a dense accumulation of glial cells along the border surrounding the cortex below the impact. (D) 60X confocal images show glial cells close together along the lesion border in a wall-like manner, suggesting gliosis. Astrocytic processes seem to interdigitate at the lesion border. MnCl₂, manganese chloride. Color image is available online at www.liebertpub.com/neu