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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Methods. 2015 Dec 17;98:26–33. doi: 10.1016/j.ymeth.2015.12.009

Figure 2. Characteristics of ligand binding with tandem Spinach2 aptamers.

Figure 2

A) Relative fluorescence yield from SPN2A and 2xSPN2A on a molar basis, B) Binding curves for SPN2A and 2xSPN2A in Buffer S, C) Binding curves for SPN2A and 2xSPN2A in Buffer IC, D) Kds of DFHBI and PFP-DFHBI for SPN2A and 2xSPN2A in buffers IC and S.