(A) Isolation of V1 and dI4/dILA interneurons. Left, interneuron populations. Middle, En1::Cre (V1) and Ptf1a::Cre (dI4/dILA) lineage-traced interneurons in p0 lumbar spinal cord. Right, FACS-isolated eYFP+ interneurons for microarray analysis.
(B) Scatter plot of expression levels of transcription factors (TFs, red) enriched in V1 interneurons from p0 mice.
(C) TFs with > 3-fold enrichment (p ≤ 0.02, one-way ANOVA) at one or more developmental ages. See also Figure S1.