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Rad6-mediated ubiquitination of H2A is inhibited by new triazine analogues. A). Western blot showing Rad6-mediated ubiquitination of histone H2A in the presence of new triazine analogues probed with ubiquitin, H2A and Rad6 antibodies. B). Represents densitometric values of western blot shown in (A), were calculated using ImageJ software and presented as relative to positive control. All experiments were repeated at least twice to verify reproducibility.
