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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Gastroenterology. 2015 Dec 8;150(4):918–930.e13. doi: 10.1053/j.gastro.2015.11.049

Figure 4. Lineage mapping of Cre-induced chief cells in Mist1-mTmG and Mist1-Kras-mTmG mice.

Figure 4

A) Experimental strategy of Cre-mediated recombination in the Mist1-Kras-mTmG mouse stomachs. The R26RmTmG/+ allele expresses red fluorescent protein in the membrane prior to Cre recombinase-mediated excision (mT). However, after Cre-dependent excision, cells express green fluorescent protein in the membrane (mG). B) Immunohistochemistry for GFP in stomach sections of control Mist1-mTmG mice (no Kras) or Mist1-Kras-mTmG mice. C) The GFP (+) metaplastic glands in the corpus were co-immunostained with antibodies against Clusterin, Muc2, or CD44v from Mist1-Kras-mTmG mouse stomachs at 2 months after tamoxifen injection. DAPI was used for nuclear staining. Boxes indicate regions enlarged. Scale bars = 100 μm.