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Light-driven hydroxylation of long chain fatty acids using Ru(II)-diimine functionalized P450 BM3 biocatalysts. The necessary electrons for the activation of molecular dioxygen at the heme active site (blue) are delivered by the Ru(II) photosensitizer covalently attached to a non-native single cysteine (L407C) in place of the reductase cofactor (FMN domain shown in light gray, PDB ID: 1BVY).
