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. Author manuscript; available in PMC: 2016 Jun 14.
Published in final edited form as: J Invest Dermatol. 2015 Dec 14;136(3):658–664. doi: 10.1016/j.jid.2015.12.006

Figure 4. IFN-γ reduces alpha toxin binding to keratinocytes and reduces ADAM10 protein levels.

Figure 4

(a) Primary keratinocytes were treated with media, IFN-γ, IL-4/IL-13 or IFN-γ in combination with IL-4/13. 50 ng/ml alpha toxin was then added for 2 hours, and cells were harvested and analyzed by western blot for alpha toxin heptamer formation. (b) Quantitation of alpha toxin binding to keratinocytes under the various cytokine conditions. (c) Cells treated with media or IFN-γ for 24 hours were harvested and analyzed for expression of ADAM10 protein. (d) Quantitation of ADAM10 protein and (e) mRNA levels in the presence and absence of IFN-γ. Data are mean ± SEM, n = 3. ***P<0.001