Skip to main content
. Author manuscript; available in PMC: 2017 Mar 24.
Published in final edited form as: Cell. 2016 Mar 24;165(1):45–60. doi: 10.1016/j.cell.2016.02.025

Figure 2. LCC cells adopt a slow-cycling state in vitro and in vivo.

Figure 2

(A) Experimental design for EdU pulse-chase experiment.

(B) Immunofluorescence images of double-positive GFP+/EdU+ LCC cells (green, red) in lungs.

(C) Quantification of double-positive EdU+/GFP+ LCC cells versus parental counterparts in lungs harvested 2 weeks post-injection. Data are mean percentage of EdU+ cells per lung ± S.E.M. N = 3 mice per group, scoring representative serial sections of the entire lung for each mouse. P < 0.05 (*), P <0.01 (**), Mann-Whitney Test.

(D) Representative immunofluorescence images of proliferating Ki-67+ (red) H2087-LCC1 (in lung) and HCC1954-LCC1 cells (in brain) 3 months post-injection.

(E) Quantification of LCC cells from Figure 2D. Data are percentage of total cells that are Ki67+ per organ ± S.E.M. N = 5 per group, scoring representative serial sections of the entire organ of each mouse. P < 0.001 (***), Student’s t-test.

(F) Micrographs of the indicated cell lines in culture 4 days post EdU labeling. White arrows point to cells retaining EdU label.

(G) Retention of eFluor670 dye by indicated cell lines after 6 days in MRM or MLM culture conditions.

(H) Cell cycle analysis of the indicated cell lines by BrdU/APC after 3 days in MRM or MLM culture conditions.

Scale bars, 10μm (B and D), and 15μm (E). See also Figure S2.