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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: J Nutr Biochem. 2015 Dec 2;30:14–23. doi: 10.1016/j.jnutbio.2015.11.001

Fig. 3.

Fig. 3

Formulation screening based on the binding affinity to and uptake of NBD-labeled void nanoparticles (Vnano) by THP-1 derived macrophages in vitro. The following parameters were changed: weight ratios of kolliphor HS15 to PC from 0.5/1 to 18/1 (A); weight ratios of (+)-α-tocopherol acetate to PC from 0.25/1 to 1/1 (B); the size of nanoparticles (C); mole ratios of KOdiA-PC to PC from 0/1 to 0.5/1 (D). NBD-labeled void nanoparticles are shown in green (λ of excitation is 460 nm, λ of emission is 535 nm). Cell nuclei were stained by DAPI and shown in blue (λ of excitation is 358 nm, λ of emission is 461 nm).