Table 2.
Effect of extracts of cocoa nibs roasted slates and unroasted well fermented cocoa (5–10 mg/mL) on programmed cell death in human lung cancer cell line after 48 hours.
| Stages of cell death | CT | RS (mg/mL) | UWF (mg/mL) | ||
|---|---|---|---|---|---|
| 5 | 10 | 5 | 10 | ||
| Viable cells (Annexin V−/PI−) |
95.80 ± 0.57 | 89.10 ± 2.26 | 86.73 ± 1.96 | 91.37 ± 1.33 | 72.03 ± 4.67∗ |
| Early apoptosis (Annexin V+/PI−) |
2.16 ± 1.03 | 1.80 ± 0.83 | 8.22 ± 2.48∗∗ | 3.87 ± 1.22 | 4.85 ± 2.05 |
| Late apoptosis (Annexin V+/PI+) |
0.90 ± 0.25 | 2.56 ± 1.30 | 4.92 ± 0.95∗ | 2.23 ± 0.67 | 8.13 ± 0.06∗∗ |
| Nonapoptotic cells (Annexin V−/PI+) |
1.12 ± 0.69 | 6.54 ± 0.13∗ | 0.14 ± 0.69 | 2.52 ± 0.68 | 14.93 ± 0.42∗∗ |
Results are expressed as percentage of total cells. The experiment is expressed as mean ± standard deviation; significant differences between untreated cells (CT) and cells treated with lycopene (5–10 μM) were compared by one-way ANOVA with the posttest of Tukey (∗ p < 0.05; ∗∗ p < 0.01).