Table 2.
Quantitative real-time PCR estimation of viral expression levels for SqLCV in plants infiltrated by pFGC-Rep/TrAP and pFGC-BC1.
| Rep/TrAP gene | BC1 gene | |||||||
|---|---|---|---|---|---|---|---|---|
| Ct of control | Ct of control reference | Ct of treatment | Ct of treatment reference | Ct of control | Ct of control reference | Ct of treatment | Ct of treatment reference | |
| Replica 1 | 24.26 | 16.47 | 29.28 | 16.28 | 27.07 | 16.92 | 29.41 | 16.64 |
| Replica 2 | 24.55 | 16.00 | 29.39 | 16.19 | 27.94 | 16.77 | 29.89 | 16.7 |
| Replica 3 | 24.07 | 16.38 | 29.87 | 15.98 | 27.45 | 16.89 | 29.44 | 16.82 |
| Average | 24.29 | 16.28 | 29.51 | 16.15 | 27.48 | 16.86 | 29.58 | 16.72 |
| ΔCt = Cts − Ctr∗ | 8.01 | 13.01 | 10.63 | 12.86 | ||||
| ΔΔCt = Ctc − Ctt∗ | 5 | 2.23 | ||||||
| Fold of change = 2ΔΔCt | 0.03125 | 0.213 | ||||||
| Silencing % = (1 − ΔΔCt) × 100 | 97% | 79% | ||||||
∗Cts = Ct values of sample; Ctr = Ct value of housekeeping gene for the same sample; Ctc = Ct values of control; Ctt = Ct values of treatment.