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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: J Colloid Interface Sci. 2016 Mar 5;471:48–58. doi: 10.1016/j.jcis.2016.03.007

Fig. 4.

Fig. 4

(a) Fluorescence intensity as a measure of the transfection of MC3T3-E1 cells depicted as a function of time and of different amounts of two different types of gene delivery carriers added per well (i.e., 5 × 104 cells): CaP synthesized at DS 15 and jetPRIME. (b) Viability of MC3T3-E1 cells transfected using different carriers, including CaP-pDNA-PLL at 20 ng/μl of PLL and CaP-pDNA-citrate at 10 mM of citrate. The amount of jetPRIME and CaP was normalized so as to deliver the identical amount of 0.5 μg of pDNA to each well seeded with cells. (c) Difference in the mean fluorescence intensity as a measure of the transfection of MC3T3-E1 and K7M2 cells on the 3rd day of transfection using CaP-pDNA synthesized at DS 15 and delivering 1 μg pDNA per well. Data are shown as averages with error bars representing standard deviation.