Figure 1.
Protein sequence alignment (A) and SDS-PAGE analysis (8% Tris-Glycine gel) of Hpβ3GlcNAcT (B) and NmLgtA (C). Lanes: BI, whole cell extract before induction; AI, whole cell extract after induction; L, lysate after induction; P, Ni2+-NTA column purified protein; M, protein markers (Bio-Rad precision Plus Protein Standards, 10–250 kDa).