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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Bioorg Med Chem. 2016 Mar 3;24(8):1696–1705. doi: 10.1016/j.bmc.2016.02.043

Scheme 1.

Scheme 1

Donor substrate specificity and acceptor substrate specificity studies of β3GlcNAcTs and β4GalTs, respectively. LacβMU was used as an acceptor for β3GlcNAcTs (NmLgtA or Hpβ3GlcNAcT) and the resulting trisaccharide products were used as acceptor substrates for β4GalTs (NmLgtB, Hpβ4GalT, or Bβ4GalT).