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One-pot four-enzyme synthesis of GalNAcβ1–3Galβ1–4GlcβProN3 (23A) (A) and Galβ1–3Galβ1–4Glc (25A) (B). Enzyme abbreviations: NahK, Bifidobacterium longum ATCC55813 N-acetylhexosamine 1-kinase;37 PmGlmU, Pasteurella multocida N-acetylglucosamine-1-phosphate uridylyltransferase;38 PmPpA, Pasteurella multocida inorganic pyrophosphatase;21 SpGalK, Streptococcus pneumoniae TIGR4 galactokinase;39 BLUSP, Bifidobacterium longum UDP-sugar pyrophosphorylase.40
