Table 2.
NmLgtA | Hpβ3GlcNAcT | |||||
---|---|---|---|---|---|---|
Substrate | kcat (s−1) | KM (mM) | kcat/KM (s−1 mM−1) | kcat (s−1) | KM (mM) | kcat/KM (s−1 mM−1) |
UDP-GlcNAc (1) | 0.55 ± 0.13 | 0.47 ± 0.11 | 1.16 | 0.46 ± 0.08 | 1.66 ± 0.28 | 0.68 |
UDP-GlcNTFA (2) | 0.19 ± 0.06 | 0.59 ± 0.20 | 0.32 | 0.32 ± 0.08 | 1.23 ± 0.31 | 0.26 |
UDP-GlcNAcN3 (3) | 0.15 ± 0.03 | 1.34 ± 0.24 | 0.11 | 0.29 ± 0.07 | 4.74 ± 1.09 | 0.062 |