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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: Bioorg Med Chem. 2016 Mar 3;24(8):1696–1705. doi: 10.1016/j.bmc.2016.02.043

Table 3.

Acceptor substrate specificities of β4GalTs using a sequential two-step process.a

graphic file with name nihms767956t2.jpg
# Acceptor β3GlcNAcT* NmLgtB Hpβ4GalT Bβ4GalT
1A GlcNAcβ1–3LacβMU 90–95% NmLgtA 100% 100% 90–95%
2A GlcNTFAβ1–3LacβMU 100% NmLgtA 95–100% 100% 60–65%
3A GlcNAcN3β1–3LacβMU 25–35% NmLgtA 100% 100% 35–40%
4A GlcNGcβ1–3LacβMU 55–60% Hpβ3GlcNAcT 90–95% 75–80% 0
5A GlcNAcPhβ1–3LacβMU 20–25% NmLgtA 100% 50% 20–25%
6A GlcNAcPhPhβ1–3LacβMU <5% NmLgtA 100% 0 0
8A GlcNH2β1–3LacβMU 20–25% NmLgtA 0 0 0
10A GlcNAc6NH2β1–3LacβMU 70–75% Hpβ3GlcNAcT 0 20% 0
11A GlcNAc6N3β1–3LacβMU 30–35% Hpβ3GlcNAcT 5–10% 100% 0
16A GlcNAc6Sβ1–3LacβMU 65–70% Hpβ3GlcNAcT 0 100% 0
21A Glcβ1–3LacβMU 20–25% NmLgtA 95–100% 0 0
23A GalNAcβ1–3LacβMU 80–90% NmLgtA 0 0 0
25A Galβ1–3LacβMU 40–45% NmLgtA 0 0 0
a

Yields estimated by HRMS. R= LacβMU.

*

LacβMU was used as the acceptor for β3GlcNAcT-catalyzed reactions. The trisaccharide producing reactions with a higher yield (catalyzed by either Hpβ3GlcNAcT or NmLgtA) were used for the acceptor substrate specificity studies of β-4GalTs.