Table 1.

Summary of key proteomic results*

DIGE spot#	Fold change	K-W P	Entry	Entry name	Protein name	Gene	Relevance to breast cancer and/or bone metastasis
751	2.2	.0039	P17987	TCPA_HUMAN	T-complex protein 1 subunit alpha	TCP1	Little or no relevant literature
904	2.0	.0028	P31943	HNRH1_HUMAN	Heterogeneous nuclear ribonucleoprotein H (hnRNP H)	HNRNPH1	Some literature, but not strong relevance (31)
904	2.0	.0028	Q96KP4	CNDP2_HUMAN	Cytosolic-nonspecific dipeptidase (EC 3.4.13.18)	CNDP2	Little or no relevant literature
1106	2.5	.0034	P06132	DCUP_HUMAN	Uroporphyrinogen decarboxylase (EC 4.1.1.37)	UROD	Some literature, but not strong relevance (32)
1106	2.5	.0034	P40121	CAPG_HUMAN	Macrophage-capping protein	CAPG	Significant relevant literature (33,34)
1106	2.5	.0034	P53365	ARFP2_HUMAN	Arfaptin-2	ARFIP2	Little or no relevant literature
1106	2.5	.0034	Q00577	PURA_HUMAN	Transcriptional activator protein Pur-alpha	PURA	Little or no relevant literature
1158	2.0	.0042	O14908	GIPC1_HUMAN	PDZ domain–containing protein GIPC1	GIPC1	Significant relevant literature (27,35–37)

* “Fold change” refers to the average of the relevant pair-wise comparisons possible within the dataset. Of the original 75 proteins identified with statistically significant fold changes, eight were upregulated in BM cells only (and no other cells) as shown in the table. These eight proteins were selected for further consideration by looking for literature evidence of relevance to breast cancer and/or bone metastasis as indicated in the table. K-W P value refers to the Kruskal-Wallis nonparametric analysis of variance test. Entry details from Universal Protein Resource (UniProt) tags are also shown. (NB multiple protein identifications from one two-dimensional gel electrophoresis spot are common.) DIGE = difference gel electrophoresis.