Fig. 4. Immunohistochemistry for DCX in the dentate gyrus of ZLC (A and B), ZLC-Al (C and D), ZDF (E and F), and ZDF-Al (G and H) groups. DCX-immunoreactive neuroblasts are observed in the subgranular zone (arrows) of the dentate gyrus. Note that DCX-immunoreactive neuroblasts were decreased in the ZLC-Al and ZDF-Al groups compared to the ZLC and ZDF groups, respectively. The lowest number of DCX-immunoreactive neuroblasts was observed in the ZDF-Al group. Scale bars = 100 µm (A, C, E and G), 25 µm (B, D, F and H).