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. Author manuscript; available in PMC: 2016 Mar 28.
Published in final edited form as: Methods Mol Biol. 2016;1408:303–317. doi: 10.1007/978-1-4939-3512-3_21

Fig. 2.

Fig. 2

Cell viability and expression efficiency. Flow cytometry analysis indicates that cells subjected to 24-h infection incubation show high expression efficiency and low toxicity. (a) Negative (top) and positive (bottom) control for PI stain on non-transduced cFB (positive control of PI stain was done with prolonged trypsin digestion to break cell membrane). (b) ChR2-cFB analysis (5000 events) and (c) ArchT-FB analysis (20,000 events) were acquired based on gates determined in control condition. (d) Both ChR2 and ArchT delivery at MOI2000 and 24-h incubation achieved over 70 % efficiency