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. Author manuscript; available in PMC: 2016 Mar 28.
Published in final edited form as: Methods Mol Biol. 2016;1408:303–317. doi: 10.1007/978-1-4939-3512-3_21

Fig. 4.

Fig. 4

Co-culture of ChR2-cFB and cardiomyocytes and confirmation of opsin functionality. (a) Fluorescent images of diffuse plating. Scale bar is 50 μm. (b) Panoramic macroscopic fluorescent image of patterned ChR-cFB cluster surrounded and covered by cardiomyocytes. Scale bar is 4 mm. (c) Optical activation of ChR2-cFB leads to excitation in cardiomyocytes. Blue bars indicate time of 470 nm light pulse. Optical stimulation was 10-ms pulse at 0.31 mW/mm2. Action potentials were acquired via Di4-ANBDQBS optically at 390 frames/s