FIG. 7.

Scanning ChIP assay of the human GAPD gene. (A) Locations of canonical E boxes, noncanonical E boxes, and exons in the human and mouse genomic sequences. Canonical E boxes in the mouse gene are indicated by black circles. The lead (+) or complement (−) sequences of noncanonical E boxes are also indicated. The regions that are amplified for the scanning ChIP assay are indicated by the lines above the human gene and labeled A to H. (B) The human GAPD gene was scanned by ChIP assay in the P493-6 cell system. P493-6 cells were either not treated (− Tet) or treated with tetracycline (+ Tet) for 72 h. ChIP was performed with anti-Myc or HGF, without antibody (no Ab), or mock control samples as indicated at the bottom of the graph.