Fig. 1.

Adipocyte differentiation requires protein S-nitrosylation. A: Total NO produced by differentiated adipocytes at each period (0–2 days, 3–5 days, or 6–8 days) was measured using an NO assay kit. *P < 0.05 compared with 0–2 days by t-test. C–E: ADSCs were grown in the IDXT condition and treated with the indicated concentration of SNOC (C), PTIO (D), or cPTIO (E). Lipid droplets in adipocytes were stained with Oil Red O dye and quantified. Data were normalized by setting the insulin control as 1 (mean ± SD). **P < 0.01 by t-test. ^#P < 0.05, ^##P < 0.01 compared with IDXT by t-test. Data are representative of three individual experiments. B: On day 9 following IDXT incubation, immunofluorescence staining was performed on the control and the 9 day differentiated adipocyte samples using an anti-nitrosocysteine antibody.