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. 2004 Jul;24(13):6011–6020. doi: 10.1128/MCB.24.13.6011-6020.2004

FIG. 5.

FIG. 5.

Coverage and identified peptides. (A) Sequence coverage of DEK protein phosphorylated with CK2 identified after nano-ESI-Q-TRAP measurements. Phosphorylated peptides are in the black box. Unphosphorylated peptides are in boldface and cover 33% of the whole protein. Taking phosphorylation and oxidation of methionine into account, the coverage increased to 99%. (B) Sequence coverage of DEK protein dephosphorylated with λ-phosphatase identified after nano-ESI-Q-TRAP measurements. The dephosphorylated peptides that were formerly phosphorylated are in the broken-line box. Taking into consideration the newly dephosphorylated peptides, the coverage of the protein increased to 45%. The phosphorylated peptides resistant to dephosphorylation are shown in the black box. Taking phosphorylation and oxidation of methionine into account, the coverage increased to 70%. (C) Phosphorylated peptides identified by nano-ESI-Q-TRAP.